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Level 14

Krebs cycle


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What happens to the pyruvate produced in glycolysis?
Pyruvate enters the mytochondrion and gets converted to AcetylCoA
How many AcetylCoAs are produced per molecule glucose?
Two acetylCoAs from 2 pyruvates resulting from glycolysis
What is the first step in pyruvate oxidation?
The carboxyl group is removed as CO2
What is the second step in pyruvate oxidation?
NAD+ is reduced to NADH
What is the third step in pyruvate oxidation?
an acetyl group is transfer for Coenzyme A making AcetylCoA
What are the similarities between FAD, NAD+ and CoA?
They are all ATP derived
What is the high energy bond in AcetylCoA?
The thioester -CO-S-
What does coenzymeA do for the Krebs cycle?
It is a carrier of acyl-groups.
AcetylCoA enters the Krebs cycle by...
attaching the 2C-atoms in the acyl-group to oxaloactetae to build citrate (6C-compound)
Citrate synthase synthesizes citrate by
condensing oxaloacetate with AcetylCoA
Aconitase is the second enzyme in the Krebs cycle and...
isomerizes citrate to isocitrate
Isocitrate dehydrogenase catalyzes the ...
oxidative decarboxylation of isocitrate while storing energy into NADH
Alpha-glutarate dehydrogenase is an enzyme complex which catalyzes ...
decarboxylation of alpha-ketoglutarate, reduction of NAD+ and transfer to CoA
SuccinylCoA-synthetase couples ...
the conversion of succinylCoA to succinate with the formation of ATP/GTP
How is the energy from succinylCoA harvested?
by breaking the thioester bond to build ATP/GTP
Succinate dehydrogenase is a membrane-bound enzyme complex which uses ...
FAD as oxidant for oxidation of succinate to fumarate
Fumarase catalyzes the 7th reaction in the Krebs cycle which is the...
reversible conversion of fumarate to malate
Malate dehydrogenase catalyzes ...
the oxidation of malate to oxaloacetate using the reduction of NAD+ to NADH
How many ATPs, NADH and FADH2 from one glucose going through glycolysis + Krebs?
4 ATP + 10 NADH + 2FADH2
How many ATPs, NADH and FADH2 are released in Krebs per molecule AcetylCoA which enters?
1ATP + 3NADH + 1FADH2