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What makes RNA unstable compared to DNA?
Its reactive hydroxyl group on the ribose, which invites hydrolysis reactions
What is the direction for transcription?
5'-3'
What do you need to initiate transcription?
A promoter, a start codon, ribonucleotides, an RNA polymerase
What are the important promoter regions in prokaryotes?
The -10 and -35 upstream regions.
What is the -10 upstream region and what does it do?
The -10 region is a specific sequence located 10 nucleotides upstream from transcription start. It's role is as a landing site for RNA polymerase.
What is the -35 upstream region and what does it do?
The -35 region is a specific sequence located 35 nucleotides upstream form transcription start. It's role is as landing site for sigma-factor 70.
What do Sigma factors do?
They enable specific binding of RNA polymerase to gene-promoters.
What is the -25 reagion in Eukaryotes?
The -25 region is the TATA box, where transcriptional factors will attach and signal RNA polymerase to attach as well.
Which are the 3 steps of transcription?
Initiation, elongation and termination.
What are the differences between DNA replication and transcription?
Transcription does not require primers, or a helicase to open the double helix.