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What makes RNA unstable compared to DNA?
Its reactive hydroxyl group on the ribose, which invites hydrolysis reactions
What is the direction for transcription?
What do you need to initiate transcription?
A promoter, a start codon, ribonucleotides, an RNA polymerase
What are the important promoter regions in prokaryotes?
The -10 and -35 upstream regions.
What is the -10 upstream region and what does it do?
The -10 region is a specific sequence located 10 nucleotides upstream from transcription start. It's role is as a landing site for RNA polymerase.
What is the -35 upstream region and what does it do?
The -35 region is a specific sequence located 35 nucleotides upstream form transcription start. It's role is as landing site for sigma-factor 70.
What do Sigma factors do?
They enable specific binding of RNA polymerase to gene-promoters.
What is the -25 reagion in Eukaryotes?
The -25 region is the TATA box, where transcriptional factors will attach and signal RNA polymerase to attach as well.
Which are the 3 steps of transcription?
Initiation, elongation and termination.
What are the differences between DNA replication and transcription?
Transcription does not require primers, or a helicase to open the double helix.