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Microbiology Practical VI: Urinary tract infection


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MALDI-TOF
Identification of bacterial protein (look at high score)
Disk diffusion method
Measure inhibition zone diameter and interpret with reference to see whether it is susceptible
Significant growth
≥10^5 CFU/ ml, not likely by contamination
Wet mount
To check roundish substance with nucleus (WBC) and bacteria (e.g. rods)
Clean catch method
Cleaning perineum and hold container to catch MSU for baby
Coagulase -ve staphylococcus
Staphylococcus Saprophyticus
Urine strip nitrite
enterococci, staphylococci (Staphylococcus saprophyticus), Acinetobacter, or adenovirus
Urine strip leukocyte reaction
Leukocyte esterase detection
Swarming growth on blood agar
No discrete colonies, but a layer of continuous growth (motile bacteria can swim across)
Urase test result
Alkaline -> orange to pink, pH indicator: Phenol red
Urase action
Split urea make it alkaline
Urease +ve swarming example
Proteus mirabilis from anus
CLED
Inhibit swarming growth
Risk of having bacteriuria in pregnant women
Pyelonephritis affecting pregnancy